Vol. 6, No. 2, 47-57, 2007

First simultaneous determination of size, refractive index, light scattering depolarization and fluorescence of phytoplankton cells by laser scanning flow cytometry
Luca Fiorani, Roberta Fantoni, Antonia Lai, Antonio Palucci, Konstantin A. Semyanov, Maria Sighicelli, Valeria Spizzichino and Peter A. Tarasov

Abstract
A new laser flow cytometer, based on a laser source of polarized light irradiating microscopic particles, has been developed. The unpolarized and linearly polarized scattered light, as well as the side emitted light in different spectral bands, are measured, allowing the simultaneous and real-time determination of size, refractive index, light scattering depolarization and fluorescence of each particle. Tests with aqueous samples of polystyrene spheres, fluorescent or non fluorescent, and phytoplankton cells demonstrate that the system is able to retrieve size and refractive index and that the light scattering depolarization and fluorescence measurements allows the sorting of particles otherwise indistinguishable.

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History
Submitted: 11 June 2007
Revised: 04 Oct 2007
Accepted: 04 Oct 2007
Published: 19 Oct 2007
Responsible editor: Rainer Reuter

Citation
Fiorani L, R Fantoni, A Lai, A Palucci, K A Semyanov, M Sighicelli, V Spizzichino & P A Tarasov, 2007. First simultaneous determination of size, refractive index, light scattering depolarization and fluorescence of phytoplankton cells by laser scanning flow cytometry. EARSeL eProceedings, 6(2): 47-57

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EARSeL European Association of Remote Sensing Laboratories, Strasbourg, France

   
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BIS Library and Information System, Carl von Ossietzky University of Oldenburg

 

ISSN 1729-3782